eef2 protein Search Results


eef2  (Boster Bio)
90
Boster Bio eef2
AKT-mTOR signaling pathway activation was detected in PCM, and inhibiting exosome secretion reduced inflammation in vivo. (a) The expression of PIK3K, AKT, p-AKT, p-mTOR, HSP90AA1, and <t>EEF2</t> was detected in PCM tissue sections by immunohistochemical staining. (b) Immunohistochemical staining scores were analyzed by the Mann-Whitney U test. There were no statistical differences for PIK3K and AKT in PCM tissues compared with NC tissues, while p-AKT and p-mTOR were overexpressed and HSP90AA1 and EEF2 were downexpressed in PCM tissues. ∗ p < 0.001 versus NC groups. (c) Western blots analyze changes of PI3K-Akt-mTOR pathway-related proteins. (d) Densitometric quantification for the changes of PI3K-Akt-mTOR pathway-related proteins was measured by QUANTITY ONE software. All values are expressed as the mean ± SD. ∗ p < 0.05 versus NC groups. (e, f) Pathological changes were detected by HE staining and inhibition of exosomes secretion by GW4869 significantly inhibited inflammatory cell infiltrate. Inflammatory infiltration scores were analyzed by the Mann-Whitney U test. ∗ p < 0.01.
Eef2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eef2/product/Boster Bio
Average 90 stars, based on 1 article reviews
eef2 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
recombinant human protein eef2  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology recombinant human protein eef2
( A ) Volcano plot of Taok2 knockout/ Taok2 WT phosphoproteome (left, prefrontal cortex; middle, hippocampus; right, ventral striatum). Phosphopeptides containing the TAOK2 kinase-recognition motif pTXX[K/R/H] are in purple and highlight potential direct substrates of TAOK2. Two data points are outside of axis limits. ( B and C ) Immunoblot and quantifications of phosphorylated <t>eEF2</t> (Thr 56 ) from cortical lysates from Taok2 +/+ and Taok2 −/− mice ( n = 5); * P < 0.05, SEM error bars, unpaired t test. β-Actin was used as a loading control. ( D ) Immunoblot of the TAOK2β IP from nontransfected N2a cells and N2a cells overexpressing WT TAOK2β shows the interaction of TAOK2β with eEF2. ( E ) Immunoblots of in vitro kinase assays using recombinant proteins as depicted in the figure show the direct phosphorylation of eEF2 (Thr 56 ) by TAOK2 kinase. ( F and G ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with WT TAOK2β and its respective control (cells expressing pcDNA3.1-myc-tag). n = 3 per condition from different independent transfection experiments; * P < 0.05, SEM error bars, unpaired t test. ( H and I ) Immunoblot and quantifications of phospho-eEF2 from LCLs lysates with TAOK2 mutation A135P (patient) and the nonaffected father. n = 3 per condition; ** P < 0.05; SEM error bars, unpaired t test. ( J and K ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with TAOK2βA135P and its respective control (cells expressing pcDNA3.1-myc-tag). n = 4 per condition from different independent transfection experiments; SEM error bars, ** P < 0.01 by unpaired t test. β-Actin was used as a loading control.
Recombinant Human Protein Eef2, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human protein eef2/product/MyBiosource Biotechnology
Average 90 stars, based on 1 article reviews
recombinant human protein eef2 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
eef2 protein  (CH Instruments)
90
CH Instruments eef2 protein
( A ) Volcano plot of Taok2 knockout/ Taok2 WT phosphoproteome (left, prefrontal cortex; middle, hippocampus; right, ventral striatum). Phosphopeptides containing the TAOK2 kinase-recognition motif pTXX[K/R/H] are in purple and highlight potential direct substrates of TAOK2. Two data points are outside of axis limits. ( B and C ) Immunoblot and quantifications of phosphorylated <t>eEF2</t> (Thr 56 ) from cortical lysates from Taok2 +/+ and Taok2 −/− mice ( n = 5); * P < 0.05, SEM error bars, unpaired t test. β-Actin was used as a loading control. ( D ) Immunoblot of the TAOK2β IP from nontransfected N2a cells and N2a cells overexpressing WT TAOK2β shows the interaction of TAOK2β with eEF2. ( E ) Immunoblots of in vitro kinase assays using recombinant proteins as depicted in the figure show the direct phosphorylation of eEF2 (Thr 56 ) by TAOK2 kinase. ( F and G ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with WT TAOK2β and its respective control (cells expressing pcDNA3.1-myc-tag). n = 3 per condition from different independent transfection experiments; * P < 0.05, SEM error bars, unpaired t test. ( H and I ) Immunoblot and quantifications of phospho-eEF2 from LCLs lysates with TAOK2 mutation A135P (patient) and the nonaffected father. n = 3 per condition; ** P < 0.05; SEM error bars, unpaired t test. ( J and K ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with TAOK2βA135P and its respective control (cells expressing pcDNA3.1-myc-tag). n = 4 per condition from different independent transfection experiments; SEM error bars, ** P < 0.01 by unpaired t test. β-Actin was used as a loading control.
Eef2 Protein, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eef2 protein/product/CH Instruments
Average 90 stars, based on 1 article reviews
eef2 protein - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
eef2 protein  (Medicago)
90
Medicago eef2 protein
( A ) Volcano plot of Taok2 knockout/ Taok2 WT phosphoproteome (left, prefrontal cortex; middle, hippocampus; right, ventral striatum). Phosphopeptides containing the TAOK2 kinase-recognition motif pTXX[K/R/H] are in purple and highlight potential direct substrates of TAOK2. Two data points are outside of axis limits. ( B and C ) Immunoblot and quantifications of phosphorylated <t>eEF2</t> (Thr 56 ) from cortical lysates from Taok2 +/+ and Taok2 −/− mice ( n = 5); * P < 0.05, SEM error bars, unpaired t test. β-Actin was used as a loading control. ( D ) Immunoblot of the TAOK2β IP from nontransfected N2a cells and N2a cells overexpressing WT TAOK2β shows the interaction of TAOK2β with eEF2. ( E ) Immunoblots of in vitro kinase assays using recombinant proteins as depicted in the figure show the direct phosphorylation of eEF2 (Thr 56 ) by TAOK2 kinase. ( F and G ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with WT TAOK2β and its respective control (cells expressing pcDNA3.1-myc-tag). n = 3 per condition from different independent transfection experiments; * P < 0.05, SEM error bars, unpaired t test. ( H and I ) Immunoblot and quantifications of phospho-eEF2 from LCLs lysates with TAOK2 mutation A135P (patient) and the nonaffected father. n = 3 per condition; ** P < 0.05; SEM error bars, unpaired t test. ( J and K ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with TAOK2βA135P and its respective control (cells expressing pcDNA3.1-myc-tag). n = 4 per condition from different independent transfection experiments; SEM error bars, ** P < 0.01 by unpaired t test. β-Actin was used as a loading control.
Eef2 Protein, supplied by Medicago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eef2 protein/product/Medicago
Average 90 stars, based on 1 article reviews
eef2 protein - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
endogenous eef2 proteins pulled down by biotin-labeled ac4c-oligos  (Oligos Etc)
90
Oligos Etc endogenous eef2 proteins pulled down by biotin-labeled ac4c-oligos
( A ) Volcano plot of Taok2 knockout/ Taok2 WT phosphoproteome (left, prefrontal cortex; middle, hippocampus; right, ventral striatum). Phosphopeptides containing the TAOK2 kinase-recognition motif pTXX[K/R/H] are in purple and highlight potential direct substrates of TAOK2. Two data points are outside of axis limits. ( B and C ) Immunoblot and quantifications of phosphorylated <t>eEF2</t> (Thr 56 ) from cortical lysates from Taok2 +/+ and Taok2 −/− mice ( n = 5); * P < 0.05, SEM error bars, unpaired t test. β-Actin was used as a loading control. ( D ) Immunoblot of the TAOK2β IP from nontransfected N2a cells and N2a cells overexpressing WT TAOK2β shows the interaction of TAOK2β with eEF2. ( E ) Immunoblots of in vitro kinase assays using recombinant proteins as depicted in the figure show the direct phosphorylation of eEF2 (Thr 56 ) by TAOK2 kinase. ( F and G ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with WT TAOK2β and its respective control (cells expressing pcDNA3.1-myc-tag). n = 3 per condition from different independent transfection experiments; * P < 0.05, SEM error bars, unpaired t test. ( H and I ) Immunoblot and quantifications of phospho-eEF2 from LCLs lysates with TAOK2 mutation A135P (patient) and the nonaffected father. n = 3 per condition; ** P < 0.05; SEM error bars, unpaired t test. ( J and K ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with TAOK2βA135P and its respective control (cells expressing pcDNA3.1-myc-tag). n = 4 per condition from different independent transfection experiments; SEM error bars, ** P < 0.01 by unpaired t test. β-Actin was used as a loading control.
Endogenous Eef2 Proteins Pulled Down By Biotin Labeled Ac4c Oligos, supplied by Oligos Etc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous eef2 proteins pulled down by biotin-labeled ac4c-oligos/product/Oligos Etc
Average 90 stars, based on 1 article reviews
endogenous eef2 proteins pulled down by biotin-labeled ac4c-oligos - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
eef2 expression  (Human Protein Atlas)
90
Human Protein Atlas eef2 expression
( A ) Volcano plot of Taok2 knockout/ Taok2 WT phosphoproteome (left, prefrontal cortex; middle, hippocampus; right, ventral striatum). Phosphopeptides containing the TAOK2 kinase-recognition motif pTXX[K/R/H] are in purple and highlight potential direct substrates of TAOK2. Two data points are outside of axis limits. ( B and C ) Immunoblot and quantifications of phosphorylated <t>eEF2</t> (Thr 56 ) from cortical lysates from Taok2 +/+ and Taok2 −/− mice ( n = 5); * P < 0.05, SEM error bars, unpaired t test. β-Actin was used as a loading control. ( D ) Immunoblot of the TAOK2β IP from nontransfected N2a cells and N2a cells overexpressing WT TAOK2β shows the interaction of TAOK2β with eEF2. ( E ) Immunoblots of in vitro kinase assays using recombinant proteins as depicted in the figure show the direct phosphorylation of eEF2 (Thr 56 ) by TAOK2 kinase. ( F and G ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with WT TAOK2β and its respective control (cells expressing pcDNA3.1-myc-tag). n = 3 per condition from different independent transfection experiments; * P < 0.05, SEM error bars, unpaired t test. ( H and I ) Immunoblot and quantifications of phospho-eEF2 from LCLs lysates with TAOK2 mutation A135P (patient) and the nonaffected father. n = 3 per condition; ** P < 0.05; SEM error bars, unpaired t test. ( J and K ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with TAOK2βA135P and its respective control (cells expressing pcDNA3.1-myc-tag). n = 4 per condition from different independent transfection experiments; SEM error bars, ** P < 0.01 by unpaired t test. β-Actin was used as a loading control.
Eef2 Expression, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eef2 expression/product/Human Protein Atlas
Average 90 stars, based on 1 article reviews
eef2 expression - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


AKT-mTOR signaling pathway activation was detected in PCM, and inhibiting exosome secretion reduced inflammation in vivo. (a) The expression of PIK3K, AKT, p-AKT, p-mTOR, HSP90AA1, and EEF2 was detected in PCM tissue sections by immunohistochemical staining. (b) Immunohistochemical staining scores were analyzed by the Mann-Whitney U test. There were no statistical differences for PIK3K and AKT in PCM tissues compared with NC tissues, while p-AKT and p-mTOR were overexpressed and HSP90AA1 and EEF2 were downexpressed in PCM tissues. ∗ p < 0.001 versus NC groups. (c) Western blots analyze changes of PI3K-Akt-mTOR pathway-related proteins. (d) Densitometric quantification for the changes of PI3K-Akt-mTOR pathway-related proteins was measured by QUANTITY ONE software. All values are expressed as the mean ± SD. ∗ p < 0.05 versus NC groups. (e, f) Pathological changes were detected by HE staining and inhibition of exosomes secretion by GW4869 significantly inhibited inflammatory cell infiltrate. Inflammatory infiltration scores were analyzed by the Mann-Whitney U test. ∗ p < 0.01.

Journal: Mediators of Inflammation

Article Title: Exosomes Play an Important Role in the Progression of Plasma Cell Mastitis via the PI3K-Akt-mTOR Signaling Pathway

doi: 10.1155/2019/4312016

Figure Lengend Snippet: AKT-mTOR signaling pathway activation was detected in PCM, and inhibiting exosome secretion reduced inflammation in vivo. (a) The expression of PIK3K, AKT, p-AKT, p-mTOR, HSP90AA1, and EEF2 was detected in PCM tissue sections by immunohistochemical staining. (b) Immunohistochemical staining scores were analyzed by the Mann-Whitney U test. There were no statistical differences for PIK3K and AKT in PCM tissues compared with NC tissues, while p-AKT and p-mTOR were overexpressed and HSP90AA1 and EEF2 were downexpressed in PCM tissues. ∗ p < 0.001 versus NC groups. (c) Western blots analyze changes of PI3K-Akt-mTOR pathway-related proteins. (d) Densitometric quantification for the changes of PI3K-Akt-mTOR pathway-related proteins was measured by QUANTITY ONE software. All values are expressed as the mean ± SD. ∗ p < 0.05 versus NC groups. (e, f) Pathological changes were detected by HE staining and inhibition of exosomes secretion by GW4869 significantly inhibited inflammatory cell infiltrate. Inflammatory infiltration scores were analyzed by the Mann-Whitney U test. ∗ p < 0.01.

Article Snippet: Samples were incubated with primarily indicated antibodies diluted at 1 : 100 overnight at 4°C: PI3K p85 alpha (Proteintech, 60225-1-Ig), p-AKT (Tr308) and AKT (pan) (Cell Signaling Technology), phospho-mTOR (Abcam, ab109268), HSP90AA1 (Boster, BA0369), and EEF2 (Boster, BM1733), and secondary rabbit-anti-human HistostainTM-SP Kit (SPN 9001 ZSGB-BIO) for 1 h at room temperature.

Techniques: Activation Assay, In Vivo, Expressing, Immunohistochemical staining, Staining, MANN-WHITNEY, Western Blot, Software, Inhibition

( A ) Volcano plot of Taok2 knockout/ Taok2 WT phosphoproteome (left, prefrontal cortex; middle, hippocampus; right, ventral striatum). Phosphopeptides containing the TAOK2 kinase-recognition motif pTXX[K/R/H] are in purple and highlight potential direct substrates of TAOK2. Two data points are outside of axis limits. ( B and C ) Immunoblot and quantifications of phosphorylated eEF2 (Thr 56 ) from cortical lysates from Taok2 +/+ and Taok2 −/− mice ( n = 5); * P < 0.05, SEM error bars, unpaired t test. β-Actin was used as a loading control. ( D ) Immunoblot of the TAOK2β IP from nontransfected N2a cells and N2a cells overexpressing WT TAOK2β shows the interaction of TAOK2β with eEF2. ( E ) Immunoblots of in vitro kinase assays using recombinant proteins as depicted in the figure show the direct phosphorylation of eEF2 (Thr 56 ) by TAOK2 kinase. ( F and G ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with WT TAOK2β and its respective control (cells expressing pcDNA3.1-myc-tag). n = 3 per condition from different independent transfection experiments; * P < 0.05, SEM error bars, unpaired t test. ( H and I ) Immunoblot and quantifications of phospho-eEF2 from LCLs lysates with TAOK2 mutation A135P (patient) and the nonaffected father. n = 3 per condition; ** P < 0.05; SEM error bars, unpaired t test. ( J and K ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with TAOK2βA135P and its respective control (cells expressing pcDNA3.1-myc-tag). n = 4 per condition from different independent transfection experiments; SEM error bars, ** P < 0.01 by unpaired t test. β-Actin was used as a loading control.

Journal: Science Advances

Article Title: The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation

doi: 10.1126/sciadv.adf7001

Figure Lengend Snippet: ( A ) Volcano plot of Taok2 knockout/ Taok2 WT phosphoproteome (left, prefrontal cortex; middle, hippocampus; right, ventral striatum). Phosphopeptides containing the TAOK2 kinase-recognition motif pTXX[K/R/H] are in purple and highlight potential direct substrates of TAOK2. Two data points are outside of axis limits. ( B and C ) Immunoblot and quantifications of phosphorylated eEF2 (Thr 56 ) from cortical lysates from Taok2 +/+ and Taok2 −/− mice ( n = 5); * P < 0.05, SEM error bars, unpaired t test. β-Actin was used as a loading control. ( D ) Immunoblot of the TAOK2β IP from nontransfected N2a cells and N2a cells overexpressing WT TAOK2β shows the interaction of TAOK2β with eEF2. ( E ) Immunoblots of in vitro kinase assays using recombinant proteins as depicted in the figure show the direct phosphorylation of eEF2 (Thr 56 ) by TAOK2 kinase. ( F and G ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with WT TAOK2β and its respective control (cells expressing pcDNA3.1-myc-tag). n = 3 per condition from different independent transfection experiments; * P < 0.05, SEM error bars, unpaired t test. ( H and I ) Immunoblot and quantifications of phospho-eEF2 from LCLs lysates with TAOK2 mutation A135P (patient) and the nonaffected father. n = 3 per condition; ** P < 0.05; SEM error bars, unpaired t test. ( J and K ) Immunoblot and quantifications of phospho-eEF2 from N2a cell lysates transfected with TAOK2βA135P and its respective control (cells expressing pcDNA3.1-myc-tag). n = 4 per condition from different independent transfection experiments; SEM error bars, ** P < 0.01 by unpaired t test. β-Actin was used as a loading control.

Article Snippet: One microgram from the recombinant human protein TAOK2 (amino acids 1 to 314) (Signal-Chem, T25-11G-10) or the recombinant human protein eEF2 (MyBioSource, MBS1213669) was subjected to process of denaturation, reduction, alkylation, and digestion and processed for normal proteomics as mentioned previously.

Techniques: Knock-Out, Western Blot, In Vitro, Recombinant, Transfection, Expressing, Mutagenesis